rabbit anti p p38  (Bioss)

Journal: International Dental Journal

Article Title: 4632427E13Rik Facilitates Jaw Marrow-Derived Mesenchymal Stem Cells Osteogenesis and Angiogenesis Under Hypoxia Through miR-34a-5p/Aldoa/Hif-1α Pathway

doi: 10.1016/j.identj.2025.109364

Figure Lengend Snippet: Aldoa promotes osteogenesis and angiogenesis via the ERK/Hif-1α pathway. A, Alizarin red staining (14 days) and ALP staining (7 days). B, The gene expression levels of Runx2, Alp and Ocn were determined by qRT-PCR. β-actin was used as an internal reference gene. C, Tube formation assay was performed in the presence of CM. The scale bars represent 100 μm. D, The mRNA expression levels of Vegf and CD31 were analysed by quantitative RT-PCR. β-actin was used as an internal reference gene. E, Immunofluorescence staining was performed to detect Vegf expression after being treated by CM. The scale bars represent 25 μm. F, A Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis was performed to determine the top related pathways involving these differentially expressed mRNAs. G and H, The protein expression levels of Aldoa, Hif-1α and the phosphorylated of ERK, JUK and p38 among hypoxia, normoxia and hypoxia + si-Aldoa groups were determined by western blot. Semiquantitative analysis of the p-ERK/ERK, p-JUK/JUK and p38/p-p38 ratios were shown. β-actin was used as an internal reference gene. All data were expressed as means ± SD. * P < .05, ** P < .01.

Article Snippet: Rabbit anti-Aldoa (1:1000, 11217-1-AP), rabbit anti-Hif-1α (1:1000, ab179483), rabbit anti-Runx2 (1:1000, 8486S), rabbit anti-CD31 (1:1000, 77699S), rabbit anti-Ocn (1:1000, bs-4917R), rabbit anti-Alp (1:1000, bs-1535R), rabbit anti-Vegf (1:1000, bs-1313R), rabbit anti-ERK (1:1000, bsm-33337M), rabbit anti-p-ERK (1:1000, bs-1646R), rabbit anti-p38 (1:1000, bs-0637R), rabbit anti-p-p38 (1:1000, bs-0636R), rabbit anti-JNK (1:1000, bs-2592R), rabbit anti-p-JNK (1:1000, bs-1640R), and mouse anti-β-actin (1:2000, AF7018) were purchased from Proteintech, Abcam, Cell Signalling Technology, and Bioss, respectively.

Techniques: Staining, Gene Expression, Quantitative RT-PCR, Tube Formation Assay, Expressing, Immunofluorescence, Western Blot

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-p38 MAPK Rabbit Ab , Abmart , TA4001 , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-p38 MAPK Rabbit Ab , Abmart , TA4001 , 1: 1500.

Techniques: Activity Assay

Journal: Genes & Diseases

Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

doi: 10.1016/j.gendis.2025.101862

Figure Lengend Snippet: YHD exerts anti-tumor effects on osteosarcoma (OS) cells through the PI3K/AKT and p38 signaling pathways. (A) Principal component analysis revealed a clear distinction in gene expression profiles between the control and YHD groups. (B) Volcano plot identified 3495 differentially expressed genes in the YHD group. (C) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. (D – G) Gene Set Enrichment Analysis (GSEA) of control and YHD groups. (H, I) Western blot analysis detected the effect of YHD on proteins related to the PI3K/AKT and MAPK pathways in OS cells. (J, K) After the addition of a PI3K activator and a P38 inhibitor, scratch healing assay showed that YHD inhibited the migration of OS cells. (L, M) After the addition of a PI3K activator and a P38 inhibitor, JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

Article Snippet: Cyclin B (#4138), Vimentin (#5741), N-cadherin (#13116), p38 (#9212), and p-p38 (#4511S) antibodies were purchased from Cell Signaling Technology (USA).

Techniques: Protein-Protein interactions, Gene Expression, Control, Western Blot, Migration, Staining, Membrane, Standard Deviation

Journal: Genes & Diseases

Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

doi: 10.1016/j.gendis.2025.101862

Figure Lengend Snippet: YHD inhibits the growth of OS in vivo . (A) The effect of YHD on tumorigenesis in nude mice. (B) The effect of YHD on tumor volume. (C) The effect of YHD on mouse weight. (D) Hematoxylin-eosin staining of the mouse tumor tissue. (E) Hematoxylin-eosin staining of the mouse lung tissue. (F) PCNA, Bcl-2, Vimentin, p-AKT, and p-P38 of the mouse tumor tissue were detected by immunohistochemistry. (G) Hematoxylin-eosin staining of the mouse heart and liver tissue. Data were presented as mean ± standard deviation ( n = 6).

Article Snippet: Cyclin B (#4138), Vimentin (#5741), N-cadherin (#13116), p38 (#9212), and p-p38 (#4511S) antibodies were purchased from Cell Signaling Technology (USA).

Techniques: In Vivo, Staining, Immunohistochemistry, Standard Deviation

Journal: Microbiology Spectrum

Article Title: Verapamil HCl demonstrates antiviral activity against porcine reproductive and respiratory syndrome virus by regulating Ca 2+ influx and promoting type I interferon production

doi: 10.1128/spectrum.03280-25

Figure Lengend Snippet: Verapamil HCl activates the p38/Nrf2/HO-1 cell signaling pathway. ( A ) MARC-145 cells were incubated with Verapamil HCl (80 µM) and collected at different time points (0, 30, 60, and 120 min) to detect the phosphorylated and total protein levels of p38, ERK1/2, and JNK using western blot. MARC-145 cells were incubated with a mixture of Verapamil HCl and a JNK inhibitor (SP600125), ERK1/2 inhibitor (PD98059), or p38 inhibitor (SB203580) for 2 h, and these cells were harvested to analyze Nrf2 ( B ) and HO-1 ( C ) expression using qPCR. ( D ) MARC-145 cells were pretreated with 80 µM Verapamil HCl for 2 h and then infected with 0.1 MOI PRRSV SD16 for 1 h. The protein expression levels of Nrf2 in the nuclear and cytoplasmic fractions were analyzed using western blot at 24 hpi. Lamin B1 and β-actin served as the loading controls. ( E ) Meanwhile, the amount of p-38, p-p38, and N protein expression in the total cellular fraction was also determined using western blot. MARC-145 cells were incubated with a mixture of Verapamil HCl (80 µM) and a p38 inhibitor (SB203580) for 2 h prior to infection with PRRSV SD16 (MOI = 0.1). The Nrf2 and N protein expression in these cells was analyzed at 24 hpi using qPCR ( F ) and western blot ( G ). GAPDH served as the internal control, and β-actin acted as the loading control. All the data shown are representative of three independent experiments. ** P < 0.01 and *** P < 0.001.

Article Snippet: The protein bands were transferred to polyvinylidene difluoride (PVDF) membranes, which were blocked with 5% skimmed milk powder for 1 h. Subsequently, the membranes were incubated with the indicated primary antibodies, including anti-PRRSV-1 and PRRSV-2 N protein antibodies; anti-heme oxygenase 1 (HO-1) mouse mAb (Servicebio); anti-p38/p-p38 rabbit pAb (CST, MA, USA); anti-ERK1/2 and p-ERK1/2 rabbit pAbs (Servicebio); anti-JNK1 + JNK2 + JNK3 rabbit pAb (Servicebio); anti-p-JNK mouse antibody (Santa Cruz, CA, USA), anti-Nrf2 antibody (Servicebio), anti-Keap1 rabbit pAb (Servicebio), anti-Lamin B1 rabbit pAb (Servicebio), and anti-β-actin antibody (Abcam, Cambridge, UK).

Techniques: Incubation, Western Blot, Expressing, Infection, Control

Journal: Microbiology Spectrum

Article Title: Verapamil HCl demonstrates antiviral activity against porcine reproductive and respiratory syndrome virus by regulating Ca 2+ influx and promoting type I interferon production

doi: 10.1128/spectrum.03280-25

Figure Lengend Snippet: Schematic diagram depicting the anti-PRRSV effects of Verapamil HCl. Verapamil HCl activates AMPK by promoting the expression of p-p38, which activates Nrf2 and its downstream gene HO-1. HO-1 upregulation promotes the host cellular type I IFN response and inhibits the production of pro-inflammatory factors. Meanwhile, Verapamil HCl reverses the calcium ion imbalance induced by PRRSV. Collectively, these mechanisms inhibit PRRSV infection at multiple stages.

Article Snippet: The protein bands were transferred to polyvinylidene difluoride (PVDF) membranes, which were blocked with 5% skimmed milk powder for 1 h. Subsequently, the membranes were incubated with the indicated primary antibodies, including anti-PRRSV-1 and PRRSV-2 N protein antibodies; anti-heme oxygenase 1 (HO-1) mouse mAb (Servicebio); anti-p38/p-p38 rabbit pAb (CST, MA, USA); anti-ERK1/2 and p-ERK1/2 rabbit pAbs (Servicebio); anti-JNK1 + JNK2 + JNK3 rabbit pAb (Servicebio); anti-p-JNK mouse antibody (Santa Cruz, CA, USA), anti-Nrf2 antibody (Servicebio), anti-Keap1 rabbit pAb (Servicebio), anti-Lamin B1 rabbit pAb (Servicebio), and anti-β-actin antibody (Abcam, Cambridge, UK).

Techniques: Expressing, Infection